Fig. 1.

Generation of the oGPCR-neuromap. 92 brain oGPCRs were selected from a previous study reporting GPCR tissue distribution in mice⁷. Coronal sections were prepared from 32 mouse brains, and customized probes were generated by plasmid production and probe transcription for DIG in situ hybridization (ISH), and by the manufacturer for RNAscope ISH. For both approaches, 50 sections across 9 slides were assayed for each brain oGPCR, representing ~200 ISH experiments in total. 78 oGPCRs were merited for semi-quantitative analysis (Fig. 2). For these 78 oGPCRs, ~160 experiments containing 1350 slides of 8000 sections are deposited online in a searchable website http://ogpcr-neuromap.douglas.qc.ca. 25 oGPCRs were selected (Supplementary Table 2) for profiling in the human brain. Approval was obtained from the institutional review board. Roughly 120 human samples were prepared from 4 to 13 postmortem individuals to span 14 brain regions, used for RNA preparation and quality controlled. Customized nanoString probes were designed to target the 25 oGPCR transcripts, which were quantified in all the 120 samples. Raw data from each individual sample are also deposited in the oGPCR-neuromap resource