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. 2018 Aug 27;1:124. doi: 10.1038/s42003-018-0123-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Overview of the IMISX-EP workflow for high-throughput in situ de novo phasing of in meso-grown microcrystals of membrane proteins. a IMISX crystallization, well preparation, and post-crystallization treatment, snap-cooling, and storage. b Automated grid scanning and serial data collection on hundreds of microcrystals in situ with a fast frame-rate detector. c Selection of isomorphous data sets based on intensity correlation coefficients (red line), analysis of crystal orientations (inset), and phase determination