Fig. 2.

Comparison of in vitro conversions of progesterone with CYP106A2 and different redox partner combinations. Reactions were run for 30 min while keeping the ratio of P450:ferredoxin:reductase at 1:20:2 (except for P450:FldA/Fpr = 1:50:50 and P450:YkuN:Fpr = 1:10:10); violet marks indicate polyhydroxylated progesterone products, blue marks indicate the main product 15β-OH-P, green marks indicate other monohydroxylated progesterone products (e.g., 11α-hydroxyprogesterone, 9α-hydroxyprogesterone, and 6β-hydroxyprogesterone) (Fig. 3), and orange marks indicate the remaining substrate progesterone. The relative particular product level was calculated by using the relative peak area of the specific product compared to the total peak areas of reactant and products