. 2018 Aug 6;1:103. doi: 10.1038/s42003-018-0107-6

Table 1.

Limits of detection (LODs), limits of quantification (LOQs), and absolute sensitivity of cPRISM-SRM for selected surrogate peptides

Gene	Estimated protein copies per cell^a	Surrogate peptide	Detection sensitivity
Gene	Estimated protein copies per cell^a	Surrogate peptide	LOD (cell)	LOQ (cell)	LOD (zmol)	LOQ (zmol)
EGFR	354,000	LTQLGTFEDHFLSLQR	20	20	11,761	11,761
H/K/NRAS	213,232	LVVVGAGGVGK	5	5	1771	1771
K/NRAS	177,780	SYGIPFIETSAK	1	5	295	1477
NRAS	82,045	SFADINLYR	5	5	681	681
HRAS	68,452	SFEDIHQYR	50^b	100^b	5685	11,371
ADAM17	36,080	NIYLNSGLTSTK	1	100^c	60	5993
SHC1	25,055	EAISLVCEAVPGAK	1	10	42	416

^aOur recent study for absolute quantification of EGFR pathway proteins

^bSignificant matrix interference for endogenous peptide at 1–50 cell equivalents (Supplementary Fig. 7)

^cTarget peptide fractions at 20 and 50 cell equivalents were not located for LC-SRM analysis