Table 1.
Strains, cell lines, plasmids and primers used in this study
| Strain | Description | Source |
|---|---|---|
| Escherichia coli | ||
| DH5αMCR | endA1 supE44 thi-1 λ- recA1 gyrA96 relA1 deoR ∆(lacZYA-argF) U196 Ø80 ∆lacZ∆M15mcrA ∆ (mmrhsdRMS mcrBC) | [39] |
| OP50 | Uracil-auxotrophic E. coli B strain | [40] |
| Salmonella enterica serovar Typhimurium | ||
| NCTC 12023 | Wild-type identical to ATCC 14028 | National Collection of Type Cultures (Colindale, UK) |
| Corynebacterium diphtheriae | ||
| CDC-E8392 | Wild-type, Biovar. mitis, tox+ | [41] |
| CAM-1 | CDC-E8392 DIP0733::pCR2.1 TOPODIP0733’ | [14] |
| INCA 402 | Isolate from a patient with pneumonia, biovar. Belfanti, tox− | [41] |
| Corynebacterium glutamicum | ||
| ATCC 13032 | Type strain, non-pathogenic | [42] |
| Cell lines | ||
| Detroit562 | Human hypopharyngeal carcinoma cells (ATCC CCL-2) | [43] |
| HeLa | Human cervical carcinoma cells (ATCC CCL-2) |
[44, 45] |
| Plasmids | ||
| pXMJ19 | ori colE1, oriCg, ptac, CmR | [46] |
| pXMJ19_DIP0733 | ori colE1, oriCg, ptac, CDCE8392_0678, CmR | [14] |
| pXMJ19_DIP0733-cc | pXMJ19 with 2700 bp insert of 3′-shortened CDCE8392_0678 (DIP0733 homolog without sequence for C-terminal disorder- and coiled-coil-region) from C. diphtheriae CDC-E8392 | This study |
| pXMJ19_Cg0896 | ori colE1, oriCg, ptac, Cg0896, CmR | |
| pXMJ19_Cg0896 + cc | pXMJ19 with 2693 bp insert of 3′-shortened Cg0896 (DIP0733 homolog without sequence for C-terminal disorder- and coiled-coil-region) from C. glutamicum ATCC 130323 and 268 bp insert of 5`-shortened CDB402_0643 (DIP0733 homolog sequence for C-terminal disorder- and coiled-coil-region) from C. diphtheriae INCA-402 | |
| Primers (5`- 3`) | ||
| Probe 16SrRNA | GCAGCCGCGGTAATACGTAG | This study |
| GGGCCCTAATACGACTCACTATAGGGACATCTCACGACACGAGCTG | ||
| Probe DIP0733 | CGACCCAGTGCTTAAGGCAT | |
| GGGCCCTAATACGACTCACTATACCTGTGCCTGCTTTGGACCC | ||
| Probe Cg0896 | GTACGACGGAACTGTTGAAC | |
| GGGCCCTAATACGACTCACTATAGCCTGCTTTGGACCCTGGGTC | ||
| pXMJ19_DIP0733-cc | CGCGCCTGCAGGTTGGCGACCGGTTTTACGCG | |
| CGCGCCCGGGTTACTTAGGGTCAATACCTACCT | ||
| pXMJ19_Cg0896 | CGCGCCTGCAGGTTGTCGACTGGTCTCACACC | |
| CGCGCCCGGGCTACTGTGCGGACTGGTAGC | ||
| pXMJ19_Cg0896 + cc | CGCGCCTGCAGGTTGTCGACTGGTCTCACACC | |
| CGCGCCTTAGGCTGCGATAGGGCTTCAG | ||