Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 4;20:105. doi: 10.1186/s13058-018-1020-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 4 — Molecular characterization of vMCF-7^∆Raf1 1GX cells with abrogated NOTCH3 expression. a Immunoblot assay showing expression of NOTCH3 in vMCF-7^∆Raf1 1GX and vMCF-7^∆Raf1 1GX/CRISPR-NOTCH3 cancer cells. b Densitometric analysis showing the percentage of NOTCH3 protein level in vMCF-7^∆Raf1 1GX/CRISPR-NOTCH3 cells relative to parental cells. Graph showing the average from three independent experiments (± SD). c Representative images of light microscopic analysis showing mammosphere (MPS) formation from vMCF-7^∆Raf1 1GX and vMCF-7^∆Raf1 1GX/CRISPR-NOTCH3 cells after 24 days of culture under nonadherent conditions (three serial passages). d Graphs showing the number and the size of MPS derived from vMCF-7^∆Raf1 1GX and vMCF-7^∆Raf1 1GX/CRISPR-NOTCH3 cells after 24 days of culture under nonadherent conditions (three serial passages). MPS size was quantified using National Institutes of Health ImageJ software (http://imagej.nih.gov/ij). Graphs show the average from three independent experiments (± SD)