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. 2018 Sep 4;20:105. doi: 10.1186/s13058-018-1020-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Invasive capacity of vMCF-7^∆Raf1 1GX cells with abrogated NOTCH3 expression. a In vitro real-time invasion assay of vMCF-7^∆Raf1 1GX and vMCF-7^∆Raf1 1GX/CRISPR-NOTCH3 cells stained in red with 5 μM Cell Tracker Red CMTPX. b Graph showing the average number of invasive cells from three independent experiments (± SD). c Immunoblot assay showing NOTCH3 expression in vMCF-7^∆Raf1 1GX cells infected with scramble lentivirus short hairpin RNA (lenti-shRNA; control) and lenti-shRNAs targeting NOTCH3 messenger RNA (mRNA). d Densitometric analysis showing the percentage of NOTCH3 protein level in vMCF-7^∆Raf1 1GX/shRNA-NOTCH3 cells relative to control. Graph showing the average from three independent experiments (± SD). e In vitro real-time invasion assay of vMCF-7^∆Raf1 1GX cells infected with scramble lenti-shRNAs (control) and lenti-shRNAs targeting NOTCH3 mRNA. f Graph showing the average number of invasive cells from three independent experiments (± SD)