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. 2018 Sep 4;20:105. doi: 10.1186/s13058-018-1020-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Invasive capacity of vMCF-7^∆Raf1 1GX cells expressing a green fluorescent protein (GFP)-tagged kinase Aurora kinase A (AURKA) construct. a Immunoblot assay showing expression of endogenous and GFP-tagged AURKA in vMCF-7^∆Raf1 1GX and vMCF-7^∆Raf1 1GX/CRISPR-NOTCH3 cells. b Densitometric analysis showing the percentage of endogenous AURKA protein levels in vMCF-7^∆Raf1 1GX and vMCF-7^∆Raf1 1GX/CRISPR-NOTCH3 cells relative to control. Graph shows the average from three independent experiments (± SD). c Immunoblot assay showing NOTCH3 protein levels in vMCF-7^∆Raf1 1GX and vMCF-7^∆Raf1 1GX/CRISPR-NOTCH3 cells expressing empty lentiviral vectors (control) and lentiviral GFP-tagged AURKA vectors. d Densitometric analysis showing the percentage of NOTCH3 protein levels in vMCF-7^∆Raf1 1GX and vMCF-7^∆Raf1 1GX/shRNA-NOTCH3 cells relative to vMCF-7^∆Raf1 1GX cells infected with empty lentiviral vectors (control). Graph shows the average from three independent experiments (± SD). e In vitro real-time invasion assay of vMCF-7^∆Raf1 1GX and vMCF-7^∆Raf1 1GX/CRISPR-NOTCH3 cells expressing empty lentiviral vectors (control) and lentiviral GFP-tagged AURKA vectors. f Graph showing the average number of invasive cells from three independent experiments (± SD)