Fig. 1.

Procedural schematic for measuring DNA content in live cells. Cells of interest are plated in coverglass-bottom chambered slides and are later transferred to an inverted microscope for the collection of time-lapse images. The acquisition is then paused ~ 2 h before the completion of the time-lapse experiment and Hoechst 33342 is added to the imaging medium at a concentration of 1 μg/mL, the acquisition is then resumed. At the completion of the time-lapse experiment, images are collected for Hoechst 33342 fluorescence and analyzed with the ProcessDNA algorithm. The time-lapse images are then concatenated with the analyzed images for DNA content (steps 1–6)