Fig. 2.

Determining the length of supravital dye saturation. Asynchronous cells were plated onto 8-well chambered slides and allowed 24 h to adhere. After the addition of Hoechst 33342 into the culture medium, fluorescent images were taken at 20-min intervals. a The integrated fluorescent intensity of cells with approximately 2C, 4C, and 8C DNA content are graphed over time, with error bars representing the standard deviation within groups. b Representative examples of cells with varying amounts of DNA content are presented in a time series with 20-min intervals. Located at the bottom left of each image are the integrated fluorescent units calculated at the corresponding time-point