Table 1.
The UPLC gradient and mobile phases: Acetonitrile and Q-water.
| Time (min) | 0 | 4 | 7 | 12 | 16 | 17 | 18 |
|---|---|---|---|---|---|---|---|
| Acetonitrile (%) | 15 | 18 | 20 | 28 | 35 | 15 | 15 |
| Water (%) | 85 | 82 | 80 | 72 | 65 | 85 | 85 |
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