Table 2.
List of shRNAs Used for CLIC3, NEK9, and PTDSS1
| Target gene | ID | Target sequence |
|---|---|---|
| CLIC3 | sh9123 | GCCTCGTTACAGGGAGTCCAA |
| sh9126 | GCAGGAGAAAGAGTTCAAATA | |
| NEK9 | sh10 | GCCTTGATTATTGTTGCAGTT |
| sh11 | CCGAGGAATGGAAGGTTTAAT | |
| sh12 | CCAAAGGAACTCAGACAGCAA | |
| sh13 | GTGAAGATCGTGCAAGGAATT | |
| sh14 | GTACATTTGGAGAGTGGCATT | |
| PTDSS1 | sh25 | CGAGCAGGTTAAATCTCTAAT |
| sh26 | GCTAGATCCAAATCTTCGATA | |
| sh27 | TGGACCTATGTTCGATGGTTT | |
| sh28 | GCAACAACGAAAGCCATTCTT | |
| sh29 | GACTGAGTTGAATACCTTCTT | |
| Nontargeting shRNA | ID | Oligonucleotide sequence |
| Scrambled | SHC002 | CCGGCAACAAGATGAAGAGCACCAACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTT |
Target sequences of shRNAs for gene silencing of CLIC3, NEK9, and PTDSS1, as well as the oligonucleotide sequence for a nontargeting scrambled control.