Table 5.
Top Proteins Identified in CLIC3‐His Pull‐Down
| Protein | Gene symbol | LFQ CLIC3_A | LFQ CLIC3_B | LFQ EV_A | LFQ EV_B | Number of unique peptides | Ratio LFQ CLIC3:EV |
|---|---|---|---|---|---|---|---|
| Chloride intracellular channel protein 3 | CLIC3 | 1.27E+09 | 1.19E+09 | nd | nd | 15 | 1.23E+09 |
| Serine/threonine‐protein kinase Nek9 | NEK9 | 6.31E+06 | 5.12E+06 | nd | nd | 3 | 5.71E+06 |
| 1‐Phosphatidylinositol 4,5‐bisphosphate phosphodiesterase beta‐3 | PLCB3 | 2.84E+06 | 4.29E+06 | nd | nd | 7 | 3.56E+06 |
| Aminoacyl tRNA synthase complex‐interacting multifunctional protein 1 | AIMP1 | 2.49E+06 | 3.29E+06 | nd | nd | 3 | 2.89E+06 |
| Casein kinase II subunit alpha 3; alpha 1 | CSNK2A1; CSNK2A3 | 1.70E+06 | 3.00E+06 | nd | nd | 3 | 2.35E+06 |
| 40S Ribosomal protein S28 | RPS28 | 1.64E+06 | 1.97E+06 | nd | nd | 3 | 1.80E+06 |
| Phosphatidylserine synthase 1 | PTDSS1 | 1.21E+06 | 1.73E+06 | nd | nd | 3 | 1.47E+06 |
| Nuclear pore complex protein Nup160 | NUP160 | 1.15E+06 | 1.06E+06 | nd | nd | 3 | 1.11E+06 |
Proteins identified by MS from CLIC3‐His pull‐down assay in hMSCs undergoing osteogenic differentiation. Protein lysates from osteogenically differentiated hMSCs were isolated on day 5 in cultures either overexpressing His‐tagged CLIC3 or transduced with EV control, and subjected to protein pull‐down for the His‐tagged CLIC3 and proteins binding to it. Proteins were determined by mass spectrometry measurements (n = 2). The top proteins are listed here, ranked on their ratio of CLIC3 versus control average LFQ intensity (ie, present in CLIC3 overexpressing condition and absent in the EV condition).
LFQ = label‐free quantification; EV = empty vector; nd = not detected.