Figure 3.

Effect of preemptive HDAC inhibitors on mechanical thresholds. The average 50% mechanical thresholds were determined for the ipsilateral side whisker pads of mice TIC injury with/without HDAC inhibitor treatment. Responses over time for each of the three initially matched groups (i.e. vehicle, SAHA and MS275; n = 5 per group) were compared to baseline. By day 21 and 28, the mechanical thresholds of mice with TIC injury treated preemptively with SAHA or MS275 were no longer significantly decreased compared to baseline levels and were significantly different from mice with TIC treated with vehicle. Note that symbols of significance for vehicle and MS275 groups are located below data points while those for SAHA are located above (n = 5 per group, * = vs. baseline; # = vs. TIC + vehicle; *p < 0.05; **p < 0.01; ***p <0.001; #p < 0.05; ##p < 0.01; ###p<0.001, two-way ANOVA, Tukey’s multiple comparisons test). SAHA: suberanilohydroxamic acid.