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. 2018 Jul 26;3(14):e99015. doi: 10.1172/jci.insight.99015

Figure 2. Chow diet–fed (CD-fed) ASKO mice at 22°C were hyperventilating and had greater energy expenditure (EE) than WT mice in the dark and early light phase.

Figure 2

Twelve to 15 hours of fasting eliminated and thermoneutral housing alleviated these changes. (A) Temporal changes in EE of 4-month-old mice at 22°C and 30°C. Calculated changes in EE between dark and light phase at 22°C and between 22°C and 30°C housing of both genotypes (n = 5–7/group, mean ± SEM). (B) Temporal changes in respiratory exchange ratio of 4-month-old mice at 22°C and 30°C (n = 5–7/group, mean ± SEM). (C–E) Respiratory rate (RR) (C), tidal volume (TV) (D), and minute volume (MV) (E) of 3-month-old ad libitum–fed mice and after 12–15 hours of fasting at 22°C and the calculated changes for each respiratory parameter between fasted and ad libitum fed (n = 5–8/group, mean ± SEM). (F) Temporal changes in EE of 3-month-old mice at 22°C after food (CD) deprivation from the mid-light phase (n = 5–7/group, mean ± SEM). *P < 0.05 WT versus ASKO by Welch’s unpaired t test.