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. 2018 Jul 26;3(14):e99015. doi: 10.1172/jci.insight.99015

Figure 6. Visceral adipose tissue (VAT) of 14-week HFD-fed ASKO mice responded to 16-hour fasting–induced lipolysis and thermogenic gene activation.

Figure 6

(A and B) Plasma insulin levels (blood collected from tail in the dark phase) (A) and body composition (B) of mice fed HFD for 8 weeks (n = 6/group, mean ± SEM). (C) Liver triglyceride (TG) levels of HFD-fed WT and ASKO mice in nonfasted and fasted states (n = 3–4/group, mean ± SD). (D) mRNA levels of thermogenic genes of perirenal fat of HFD-fed WT and ASKO mice (n = 3–4/group, mean ± SD). (E) Western blot analysis of p-HSL, HSL, ATGL, and actin in mesenteric fat of fasted HFD-fed WT and ASKO mice. Results presented as relative protein levels of p-HSL and ATGL after normalization to corresponding HSL and actin (n = 3/group, mean ± SD). (F) Insulin resistance determined using homeostatic model assessment (HOMA-IR) index. HOMA-IR was calculated by multiplying 16-hour fasting insulin (μU/ml) with 16-hour fasting glucose (mmol/l) and dividing by 22.5 (n = 3/group, mean ± SD). *P < 0.05, **P < 0.01, HFD-fed ASKO versus HFD-fed WT by Welch’s unpaired t test (A, B, E, and F). In C and D, data of ad libitum HFD-fed mice are also shown in Figure 4, A and G.