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. 2018 Jul 26;3(14):e121370. doi: 10.1172/jci.insight.121370

Figure 4. Characterization of DRAinh-A250 action.

Figure 4

(A) Concentration dependence of DRAinh-A250 inhibition of slc26a3-mediated Cl–/HCO3– exchange (mean ± SEM, n = 6). Inset: original BCECF fluorescence curves for Cl–/HCO3– exchange. (B) Concentration dependence of DRAinh-A250 inhibition of slc26a3-mediated Cl–/SCN– exchange (mean ± SEM, n = 6). (C) Time course of DRAinh-A250 (1 μM) inhibition of slc26a3-mediated Cl–/I– exchange (mean ± SEM, n = 4). Inset: reversibility of DRAinh-A250 (1 μM) inhibition of slc26a3-mediated Cl–/I– exchange (data representative of 4 replicates). (D) Inhibition of (human) SLC26A3-mediated Cl–/I– exchange in HEK cells. YFP fluorescence traces (top) and summary data (mean ± SEM, n = 12–26 individual cells analyzed) (bottom).