Figure 5. DRA_inh-A250 selectivity.

(A) Time course of YFP fluorescence in Cl^–/I^–-exchange assays for pendrin (slc26a4), PAT-1 (slc26a6), slc26a9, CFTR (forskolin [fsk] stimulated), and TMEM16A (ATP-stimulated) in the absence (black lines) and presence (gray lines) of 10 μM DRA_inh-A250. See Methods for assay details. (B) Summary data for studies as in A with data normalized to control conditions (white bars) (mean ± SEM, n = 6 for plate reader assays, and n = 10–28 for single cell assays, differences not significant by two-tailed t test). (C)Left panel: short-circuit current (I_sc) in well-differentiated HBE cells grown at an air-liquid interface. Studies done in the absence (black traces) and presence (gray traces) of 10 μM DRA_inh-A250. Where indicated, amiloride (20 μM), forskolin (20 μM), CFTR_inh-172 (10 μM), and ATP (100 μM) were added. Right panel: changes in I_sc for modulator additions (mean ± SEM, n = 3, differences not significant by two-tailed t test).