(A) Effects of DRAinh-A250 (10 μM) and tenapanor (10 μM), individually and together, on loop weight/length ratio (top) and luminal pH (bottom) in mouse distal colonic closed loops (mean ± SEM, n = 4 loops per group). (B) Effects of DRAinh-A250 (10 μM) and tenapanor (10 μM), individually and together, on loop weight/length ratio (top) and luminal pH (bottom) in mouse midjejunal closed loops (mean ± SEM, n = 5–9 loops per group). *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA with post-hoc Newman-Keuls multiple comparisons test. DRAinh, DRAinh-A250; Ten, tenapanor.