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. 2018 Jun 7;3(11):e120137. doi: 10.1172/jci.insight.120137

Figure 2. Effect of pirfenidone on myocardial inflammation (day 4) after DT treatment.

Figure 2

Mice expressing the diphtheria toxin receptor (DTR) in the myocardium were exposed to diphtheria toxin (DT) and fed either chow enriched with pirfenidone (DTR-PFD) or regular chow (DTR-control). Mice were sacrificed at day 4 after DT injection and the heart was collected for analysis via flow cytometry. (A) Total number of CD45+ cells/mg heart tissue (n = 17 control, n = 19 pirfenidone). (B) Leukocyte subsets in the myocardium (percentage of total: CD19+, n = 14 control, n = 16 pirfenidone; Ly6g+, n = 6/group, Ly6C+CD64lo/–, n = 10 control, n = 12 pirfenidone; CD64+Ly6Clo/–, n = 10 control, n = 12 pirfenidone). (C) Representative FACS analysis of MHC-II and CCR-2 macrophages and monocytes. (D) Macrophage/monocyte subsets in the myocardium as defined by expression of CCR2, low (l) or high (h) and MHC-II expression, low (l) or high (h). Percentage of total, n = 10 control, n = 12 pirfenidone.*P < 0.05. Bars represent the mean, and error bars represent standard deviation. P values were calculated with Student’s t test.