Table 1.
Characterization of the synthesized polycations
| Polycations | R1:R2 | CBA percentage | Mwa | PDIa | Buffering capacityb (%) |
|---|---|---|---|---|---|
| pAPOL-1 | 0:1 | 100% CBA | 1,420 | 1.1 | 82 |
| pAPOL-2 | 1:2 | 67% CBA | 3,080 | 1.6 | 38 |
| pAPOL-3 | 2:1 | 33% CBA | 2,375 | 1.4 | 39 |
| dPEI | – | – | 3,050 | 1.8 | 38 |
| bPEIc | – | – | 25,000 | 50 |
Notes:
a
Mw and PDI were determined by SEC.
b
Buffering capacity of polycations ranged between pH 5.1 and 7.4 in 150 mM NaCl.
c
The absolute Mw of branched PEI is 25 kDa.
Abbreviations: CBA, N,N′-cystaminebisacrylamide; Mw, weight-average molecular weight; PDI, polydispersity index; dPEI, degradable polyethylenimine; bPEI, branched polyethylenimine; SEC, size exclusion chromatography; pAPOL, poly(amino pentanol).