Figure 1.

WZ35 inhibits cell growth and induces apoptosis in H1975 cells. (A) Chemical structures of compound WZ35. (B) The effects of WZ35, erlotinib, and gefitinib on the viability of H1975 cells. H1975 cells were incubated with increasing concentrations of WZ35, erlotinib, and gefitinib (0.625–20 μM) for 24 h. Cell viability was determined by MTT assay, and the IC₅₀ values were calculated. (C) Apoptosis in H1975 cells was determined by flow cytometry after treating cells with WZ35 for 24 h. (D) Quantification of data presented in panel D (*P<0.05, **P<0.01, and ***P<0.001 compared with DMSO control). (E–I) H1975 cells were treated with WZ35 for 20 h. Cell lysates were subjected to Western blot analysis for apoptosis-related proteins, cleaved-PARP (E), Bax (F), Bcl-2 (G), Pro-caspase 3 (H), and Cle-caspase 3 (I). GAPDH was used as internal control.

Abbreviations: DMSO, dimethyl sulfoxide; PI, propidium iodide; FITC, fluorescein isothiocyanate; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.