Figure 2.

WZ35 induces cell cycle arrest in H1975 cells. (A) Induction of cell cycle arrest in H1975 cells was determined by PI staining and flow cytometry, after treating cells with WZ35 for 18 h. (B) The distribution of cell cycle phase in cells treated with WZ35. (C) Representative histograms from flow cytometry analysis in the three TNBC cells treated with osthole. (*P<0.05 compared with DMSO control). (D) Expression of cell cycle-associated proteins showing decreased levels of MDM2, cyclin B1, and CDC2 following treatment of cells with WZ35 for 18 h. GAPDH was used as internal control. (E) Western blot results from (D) was calculated and represented as the percent of control. (*P<0.05 and ***P<0.001 compared with DMSO control).

Abbreviations: DMSO, dimethyl sulfoxide; PI, propidium iodide; MDM2, murine double minute; CDC2, cyclin-dependent kinase 1.