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. 2018 Jul 12;3(13):e96672. doi: 10.1172/jci.insight.96672

Figure 4. Sequential Notch activity from endocardium to trabecular grooves in the WT zebrafish embryo.

Figure 4

Our 4D LSFM imaging captured sequential Notch1b activation (green) from endocardium to epicardium in the transgenic Tg(Tp-1:GFP;cmlc:mcherry) line. (A–E) At 3 dpf, Notch1b activity localized to the endocardial layer and AV canal. (F–J) At 4 dpf, Notch activity located primarily in the epicardium versus in the endocardium. Epicardial Notch1b activity and trabecular ridges organized into an alternating pattern. (K–O) At 5 dpf, trabeculae developed into a network structure. Notch activity was prominent in both the endocardium and trabecular grooves. Red scale bar: 50 μm. Green scale bar: 10 μm. Dotted lines in E, J, and O indicate endocardium. Solid lines in E, J, and O indicate epicardium. n = 3.