Fig. 1.

The kinetic changes of cAMP levels in both oocyte and cumulus cells, and phosphorylation of Cx43 in FSH-induced mouse oocyte meiotic resumption. (A-C) COCs were in vitro cultured with or without 0.05 IU/ml FSH in HX-media for 24 h (A) or with FSH for 10 h (B,C). (A) For evaluating the oocyte meiosis kinetic changes, the rate of GV stage oocyte was scored at 4 h intervals during the 24 h culture. Data was represented as mean percentage of GV±s.e.m. of three independent experiments. **P<0.01, compared with each corresponding TP in the control group. For detecting cAMP kinetic changes in response to FSH stimulation, oocytes (B) and cumulus cells (C) were respectively collected at various TPs during 10 h culture for RIA. The means±s.e.m. of the results of three individual experiments were presented, different letters indicate P<0.05 (t-test) to 0 h. (D) The effect of FSH on pCx43. Cumulus cells from 50 COCs treated with or without 0.05 IU/ml FSH in HX-media were collected at various TPs during 24 hs in vitro culture period for immunoblotting. The experiments were performed three times with similar results. *** indicates P<0.001 (t-test), compared to 0 h.