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. 2018 Jul 30;7(8):bio034678. doi: 10.1242/bio.034678

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — The effect of PKCε specific inhibitor on both FSH-induced meiotic resumption and Cx43 phosphorylation in cumulus cells of COCs. (A) When COCs were in vitro cultured, different concentrations of specific inhibitor PKCε and εV1-2 (1-100 μM) were respectively added into HX-media containing 0.05 IU/ml FSH. The percentage of GV was recorded at 24 h of culture. Different letters on each column indicate P<0.05 compared to the control, respectively. (B) Cumulus cells from 50 COCs treated with or without 50 μM εV1-2 in HX-media containing 0.05 IU/ml FSH were collected at various TPs for immunoblotting. The experiments of each assay were performed three times with similar results. *** indicates P<0.001 compared to the control of the same group.