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. Author manuscript; available in PMC: 2018 Nov 16.
Published in final edited form as: Nat Chem Biol. 2018 May 16;14(6):618–626. doi: 10.1038/s41589-018-0063-y

Figure 3. [Cy5]tRNAPhe dwell time is longer on slow ribosomes.

Figure 3

a MSD-estimated apparent diffusion coefficients of [Cy5]tRNAPhe in streptomycin pseudodependent CH2273 cells (SmP, n = 15,571), and the CH2273 genetic background strain, MG1655 (n = 10,239). Data for [Cy5]tRNAPhe in DH5a cells (Fig. 1e) is shown for comparison. b HMM estimated dwell times for [Cy5]tRNAPhe in the slow state (see Supplementary Table 1). The trajectories used were the same as in panel a. Error bars represent bootstrap estimates of standard errors.