Fig. 1.

MICU3 is a tissue-specific MCU modulator. a Multiple alignment of human MICU1, MICU2 and MICU3 protein sequences. Identical and similar residues are highlighted in dark and light blue respectively. The predicted mitochondrial targeting signal is shown in red. EF hand domains are shown in orange. The conserved cysteine involved in dimerization is shown in yellow. b RNA was extracted from Tibialis Anterior (Skeletal muscle), cardiac ventricles (Heart), liver and whole brain of C57BL/6 mice and real-time PCR of MICU1, MICU2 and MICU3 mRNAs was performed. The data were normalized to the expression levels in skeletal muscle and are shown as the average ± s.d. of two different animals. c HeLa cells were transfected with the indicated constructs and harvested after 48 h. Total proteins were extracted from cells or homogenized brains and subjected to western blotting analysis with MICU1 and MICU3 antibodies. SDS-PAGE was performed in the absence of DTT, except where indicated. *indicates non-specific bands