Table 1. Centrifugation parameters used for separation of RBCs from whole blood prior to subsequent ATP transduction experiments.

Experimental method	Centrifugation Parameters			Experimental ATP Values			Citation
	Force (g)	Duration (min)	Repet-itions	Blood type	Hct (%)	[ATP] (μM)
Filter paper	1000	10	4	Human	10	1–4	Sprague et al., 1996 [2]
	1000	10	4	Human	10	0.5–2	Sprague et al.,1998 [16]
	500	10	4	Human	10	0.24–0.75	Sprague et al., 2001 [17]
Microbore tubing	500	10	4	Rabbit	7	0.4–7	Edwards et al., 2001 [7]
	500	10	4	Rabbit	7	3–10	Sprung et al., 2002 [8]
	500	10	4	Rabbit	7	~0.7–6	Fischer et al., 2003 [14]
	500	10	4	Rabbit	7–10	~6±2	Sprague et al., 2003 [6]
	500	10	4	Human	7	0.19±0.1	Carroll et al., 2006 [19]
	500	10	4	Human	7	0.21±0.07	Subasinghe et al., 2008 [18]
	2000	10	1	Human*	7	~0.05–0.13	Wang et al., 2014 [23]
Microchannels	500	10	4	Rabbit	7	2.5–24	Price et al., 2004 [9]
	500	10	4	Rabbit	7	0.3–0.7	Price et al., 2006 [10]
	500	10	4	Rabbit	7	0.5–7	Moehlenbrock et al., 2006 [11]
	N.R.	5	4	Human	10	0.2–1.6	Wan et al., 2008 [12]
	N.R.	N.R.	N.R.	Human	10	0.6–0.8	Cinar et al., 2015 [13]
Vortexing	500	10	4	Human	10	Rel. Inc.†	Sikora et al., 2014 [24]
Viscometer	135	4	3	Human	40	Rel. Inc.	Forsyth et al., 2011 [15]
Hypoxia	500	10	4	Human	20	Rel. Inc.	Sridharan et al., 2010 [21]
	833	5	3	Human	0.1	Rel. Inc.	Bergfeld et al., 1992 [4]
Hypoxia + deformation	500	10	4	Rabbit	7	0.1–1.6	Faris et al., 2008 [5]

ATP concentrations are only included here for healthy untreated controls.

*Indicates the use of RBCs stored for 1–36 days treated using standard protocols.

^†Indicates researchers who do not report absolute ATP concentrations but who do report a relative increase compared to the control.