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. 2018 Aug 30;11:5269–5278. doi: 10.2147/OTT.S168654

Figure 4.

Figure 4

miR-132 mediates the tumor-suppressive effects of SOX2OT knockdown in NSCLC cells.

Notes: (A) Relative expression of miR-132 was examined by qRT-PCR and normalized to U6 expression in A549 and H1299 cells transfected with nontargeting sequences (sh-NC), short-hairpin RNA against SOX2OT (sh-SOX2OT), and sh-SOX2OT + miR-132 inhibitor. (B–E) Cell proliferation, migration, and invasion were determined in A549 and H1299 cells transfected with sh-NC, sh-SOX2OT, and sh-SOX2OT + miR-132 inhibitor. (F) E-cadherin, N-cadherin, and vimentin levels were determined by Western blot in A549 and H1299 cells transfected with sh-NC, sh-SOX2OT, and sh-SOX2OT + miR-132 inhibitor. *P<0.05 and **P<0.01.

Abbreviations: CCK8, cell counting kit-8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; miR-132, microRNA 132; NSCLC, non-small-cell lung cancer; qRT-PCR, quantitative real-time polymerase chain reaction; SOX2OT, Sox2 overlapping transcript.