Figure 1.

Principle of CLE. (A) Simplified scheme of probe-based CLE and principle of 2D image formation due to sweeping scanning movement of the laser beam. (B) Image representing the Z-stack formation principle. Individual images of a tumor taken with CLE are combined into a Z-stack. The 3 µm between the images corresponds to a Z step; 30 µm is the total scanned depth.

Note: Used with permission from Barrow Neurological Institute.

Abbreviations: 2D, two-dimensional; CLE, confocal laser endomicroscopy.