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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Acta Biomater. 2018 Jun 22;76:193–207. doi: 10.1016/j.actbio.2018.06.029

Fig. 9.

Fig. 9.

Mean cytokine levels in the supernatants of spleens collected from mice in the therapeutic study. At day 18, mice were sacrificed and splenocytes were restimulated with 20 μg/mL of Melan-A:26 isolated or combined with gp100:44 antigens for five days. The levels of the cytokines (A) TNF-α, (B) IFN-γ and (C) IL-4, were measured in culture supernatants by Quantikine®ELISA. Mean ± SD; n = 3, where n stands for the number of measurements per experiment. Statistics: Independent samples T-test; *p < 0.05,**p < 0.01 and ***p < 0.001.