Table 2.

Physicochemical properties of NP formulations. Mean ± SD; N = 3, n = 3, where N denotes the number of independent experiments and n denotes the number of measurements per experiment.

Formulations	Z-Aved (nm)	PdIe	ZPf (mV)	EEg (%)			LCh(¼g/mg) (%)
				Antigens	CpG	MPLAi	Antigens	CpG	MPLAi
NP[Ma]	134 ± 6.1	0.067 ± 0.024	−6.30 ± 0.80	57.8 ± 7.12	64.67 ± 3.67	99.97	14.42 ± 3.43	2.33 ± 0.13	3.00
NP[M+Gb]	138 ± 4.7	0.072 ± 0.021	−4.57 ± 0.65	53.1 ± 10.08	83.49 ± 0.41	99.96	26.56 ± 5.04	5.43 ± 0.03	4.80
NP[M+G+GalCerc]	138 ± 6.1	0.159 ± 0.038	−4.17 ± 1.20	51.98 ± 0.78	78.71 ± 4.90	99.94	25.99 ± 0.39	5.12 ± 0.32	4.80

^aMelan-A (26–35(27L), sequence ELAGIGILTV).

^bgp100 (44–59), sequence WNRQLYPEWTEAQRLD.

^cα-Galactosylceramide.

^dHydrodynamic mean diameter (Z-ave).

^ePolydispersity index (PdI).

^fZeta potential (ZP).

^gEntrapment efficiency (EE).

^hLoading capacity (LC).

ⁱMPLA content determined by LAL Chromogenic Endotoxin Quantitation Kit^® (SD is zero due to high loading values and kit sensitivity).