Stimulus parameters applied to the ON to induce temporal phase shifting and peptide release in the SCN and outcomes.
(A) Three different 5-min electrical stimulation paradigms were utilized: Orange, 1 V, 10 Hz, 0.2 ms pulse
duration; Green, 5 V, 5 Hz, 1 ms pulse duration; Purple, 5 V, 20 Hz, 1 ms pulse duration. (B) These three stimulation
parameters at distinct temporal windows caused different patterns of peptide release (■), and phase shifts of a delay of
~ 2 hr (φD), advance of ~2–3 hr (φA), or an advance of ~ 6 hr (φA+),
respectively. N.R., no releasate measured.