Table 1. Peptides detected directly from stimulated releasate of the suprachiasmatic nucleus.
| Putative Peptide (Prohormone) Name | Observed m/z (MH+) | Theoretical m/z (MH+) | Ref | RHT Stimulus-Triggered Releasate Conditions | ||||
|---|---|---|---|---|---|---|---|---|
| Pre-Stim | CT6 | CT14 | CT19 (5Hz) | CT19 (20Hz) | ||||
| Angiotensin I (Angiotensinogen) | 1296.62 | 1296.69 | 38 | • | ||||
| Little SAAS (Pro-SAAS) | 1784.97 | 1784.98 | 23, 38–39 | • | • | |||
| Big LEN (Pro-SAAS) | 1745.95 | 1745.97 | 38 | • | ||||
| PEN (Pro-SAAS) | 2301.25 | 2301.24 | 38–39 | • | • | • | • | |
| Cerebellin, aa57–71 (Cerebellin-1) | 1495.75 | 1495.79 | 23 | ‡ | • | • | • | |
| Galanin (Galanin) | 3163.59 | 3163.58 | 23, 38 | • | • | • | • | |
| P-endorphin (POMC) | 3463.80 | 3463.86 | 61 | • | ||||
| Melanotropin α (POMC) | 1622.78 | 1622.79 | 38, 40 | • | ||||
| Morphogenetic Neuropeptide (HA) | 1141.68 | 1141.67 | 61 | • | ||||
| Neurokinin-B | 1210.56 | 1210.54 | 38 | • | ||||
| Pro-enkephalin A, aa219–229 (PENK) | 1466.64 | 1466.65 | 23, 38, 40 | • | ||||
| ProSomatostatin 89–100 | 1244.58 | 1244.57 | 38 | • | ||||
| Somatostatin 14 | 1637.73 | 1637.71(ss) | 38 | • | ||||
| Substance P (Protachykinin-1 (PPT)) | 1347.73 | 1347.74 | 23, 38, 40 | • | ||||
| Thymosin β−4 (Thymosin β−4) | 4961.50 | 4961.49(a) | 38 | ‡ | • | • | • | • |
| Arginine-Vasopressin (AVP-NPII) | 1084.46 | 1084.46(ss) | 38, 40 | ‡ | • | • | • | • |
| Vasopressin-Neurophysin2-copeptin, aa151–168 (AVP-NPII) | 1948.00 | 1948.01 | 23 | • | ||||
| Vasopressin-Neurophysin2-copeptin, aa154–168 (AVP-NPII) | 1607.80 | 1607.80 | 23 | • | ||||
| MBP, aa2–18 (MBP) | 2028.10 | 2028.07 (a) | 39 | • | • | |||
| Secretogranin 2, aa598–612 [Secretogranin 2] | 1785.81 | 1785.85 | 23 | • | • | |||
‡
, Corresponding peaks identified in < 50% of the samples taken for the particular Releasate Condition. (ss), mass w/disulfide bond; (a), mass w /acetylation; (p) mass w/pyro-glu PTM at Q