Table 1.

Summary of the multigene tests

Test name	Samples	Key references	Method	Genes No.	Genes
Oncotype DX	FFPE tumor tissue	[31,32]	QRT-PCR	16 + 5	MKI67, AURKA, BIRC5, CCNB1, MYBL2, ERBB2, GRB7, ESR1, PGR, BCL2, SCUBE2, MMP11, CTSL2, GSTM1, CD68, BAG1 (+ ref genes ACTB, GAPDH, RPLPO, GUS, TFRC)
MammaPrint	Fresh or freshly frozen breast cancer tissue or FFPE tissue	[38-40]	DNA microarray	70	AA555029_RC, ALDH4A1, AP2B1, AYTL2, BBC3, C16orf61, C20orf46, C9orf30, CCNE2, CDC42BPA, CDCA7, CENPA, COL4A2, DCK, DIAPH3, DTL, EBF4, ECT2, EGLN1, ESM1, EXT1, FGF18, FLT1, GMPS, GNAZ, GPR126, GPR180, GSTM3, HRASLS, IGFBP5, JHDM1D, KNTC2, LGP2, LIN9, LOC100131053,LOC100288906, LOC730018, MCM6, MELK, MMP9, MS4 A7, MTDH, NMU, NUSAP1, ORC6L, OXCT1, PALM2, PECI, PITRM1, PRC1, QSCN6L1, RAB6B, RASSF7, RECQL5, RFC4, RTN4RL1, RUNDC1, SCUBE2, SERF1A, SLC2A3, STK32B, TGFB3,TSPYL5, UCHL5, WISP1, ZNF533
Endopredict	FFPE tumor tissue	[41,42]	QRT-PCR	8 + 4	BIRC5, UBE2C, DHCR7, RBBP8, IL6ST, AZGP1, MGP, STC2 (+ ref genes CALM1, OAZ1, RPL37A, HBB)
Prosigna (based on PAM50)	FFPE tumor tissue	[33-37]	Nanostring	50 + 8	MIA, SFRP1, KRT14, KRT17, KRT5, FGFR4, GRB7, ERBB2, BAG1, MDM2, ACTR3B, BLVRA, CXXC5, TMEM45B, MMP11, FOXC1, EGFR, CDH3, PHGDH, MYC, CCNE1, CDCA1, CDC20, KIF2C, TYMS, KNTC2, UBE2T, MELK,PTTG1, CCNB1, CDC6, MYBL2, BIRC5, CENPF, EXO1, ORC6L, ANLN, UBE2C, RRM2, MKI67, CEP55, PGR, NAT1, SLC39A6, BCL2, ESR1, MAPT, GPR160, MLPH, FOXA1 (+ 8 ref genes)

FFPE: Formalin-fixed paraffin-embedded; qRT-PCR: Quantitative reverse transcriptase-PCR.