Table 2.
Conventional analysis of diagnostic accuracy using positivity of MAT or culture (n = 33) as the reference standard
| Sample type | PCR | Reference standard |
Specificity % (95% CI) | Sensitivity % (95% CI) | |
|---|---|---|---|---|---|
| Positive (n = 33) | Negative (n = 754) | ||||
| Serum (n = 766) | rrs | 3 | 11a | 9.38 (1.98–25.0) | 98.5 (97.3–99.3) |
| PHE | 3 | 9a | 9.38 (1.98–25.0) | 98.8 (97.7–99.4) | |
| Buffy coat (n = 750) | rrs | 1 | 7a | 3.03 (0.08–15.8) | 99.0 (98.0–99.6) |
| PHE | 4 | 7a | 12.1 (3.4–28.2) | 99.0 (98.0–99.6) | |
| Urine (n = 626) | rrs | 5 | 59a | 17.2 (5.85–35.8) | 90.1 (87.4–92.4) |
| PHE | 4 | 6a | 13.8 (3.89–31.7) | 99.0 (97.8–99.6) | |
a
Number of samples negative by the reference standard but positive by PCR that had paired MAT samples: serum: rrs (n = 6), PHE (n = 5); buffy coat: rrs (n = 5), PHE (n = 3); urine: rrs (n = 23), PHE (n = 1).