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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Curr Protoc Chem Biol. 2018 Aug 7;10(3):e43. doi: 10.1002/cpch.43

Figure 1. T-REX outline.

Figure 1

A) Outline of T-REX workflow. Upon light shining, reactive signal (HNE, red dot) is released and reacts with the POI due to proximity. Analyses include calculating targeting efficiency and modification site determination. B) Chemical structure of unbound Ht-PreHNE (left) and Ht-PreHNE bound to HaloTag after light shining (middle). The HNE(alkyne) released (right) upon light shining is shown as red circle. C) The generic LDE structure as well as several LDEs that have been used with T-REX are shown. D) An illustrative model of N-terminal HaloTagged POI with the photocaged-precursor, Ht-PreHNE, bound at Halo. Ribbon structure is adapted from PDB:1J7D. The conditions used for photouncaging of the LDE (red dot) are shown.