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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Curr Protoc Chem Biol. 2018 Aug 7;10(3):e43. doi: 10.1002/cpch.43

Figure 2. Plasmids Construction and Design.

Figure 2

A) pCS2+8-HaloTevKeap1HA plasmid used for T-REX, includes an HA epitope tag on Keap1, allowing for enhanced detection during analysis. B) T-REX split control 1: pCS2+8-HaloP2AKeap1HA uses P2A, a self-cleaving linker, to produce HaloTag and Keap1 separately. C) T-REX split control 2: Two plasmids (pCS2+8-Halo and pCS2+8-Keap1), each expressing either HaloTag or POI, are cotransfected into the system, leading to separately expressed HaloTag and Keap1. D) Outline of various built-in controls of T-REX along with expected outcomes of each control.