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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Curr Protoc Chem Biol. 2018 Aug 7;10(3):e43. doi: 10.1002/cpch.43

Figure 5. Worm data.

Figure 5

A) Biotin pulldown data from C. elegans showing enrichment of Keap1 upon T-REX treatment in live worms. In this experiment Ht-PreHNE with no alkyne handle was used as a negative control. After T-REX treatment, lysates were Clicked with biotin azide and enriched with a streptavidin pulldown. The elution shows enrichment with T-REX conditions compared to the negative controls. B) Quantification of enrichment from the various conditions. Adapted with permission from http://pubs.acs.org/doi/abs/10.1021%2Facs.biochem.7b00642.