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. Author manuscript; available in PMC: 2019 Sep 15.
Published in final edited form as: J Immunol. 2018 Jul 30;201(6):1692–1704. doi: 10.4049/jimmunol.1800244

FIGURE 2.

FIGURE 2.

Cebpa enhancer deletion using Mb1-Cre does not reduce marrow proB, early preB, or preB cells. (A) Diagram of breeding strategy to obtain Cebpa Enh(f/f);Mb1-Cre mice (left). Examples of WT versus floxed Cebpa enhancer and Mb1-Cre PCR analysis - mouse 6 (underlined) has genotype Enh(f/f);Mb1-Cre (right). (B) RNA from flow-sorted GMP, preproB, proB, and preB cells from WT mice was analyzed for Mb1 mRNA expression relative to mS16 mRNA, with expression in preB cells set to 1.0 (left, mean and SD from three determinations). Genomic DNA from preproB, proB, early preB, and preB cells from two Enh(f/f) and two Enh(f/f);Mb1-Cre mice were analyzed for retention of the floxed Cebpa enhancer by PCR followed by agarose gel electrophoresis and ethidium bromide staining (right). (C) Total bone marrow mononuclear cells, Lin-Sca-1+c-kit+ (LSK) cells, GMP, CLP, preproB, proB, early preB, and preB cells were enumerated in Enh(f/f) and Enh(f/f);Mb1-Cre littermates (mean and SD from three determinations).