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. Author manuscript; available in PMC: 2019 Sep 15.
Published in final edited form as: J Immunol. 2018 Jul 30;201(6):1692–1704. doi: 10.4049/jimmunol.1800244

FIGURE 3.

FIGURE 3.

Splenic B cells lacking the Cebpa +37 kb enhancer retain normal immunoglobulin heavy chain class switching. (A) Genomic DNA from CD43- splenic B cells from two Enh(f/f) and two Enh(f/f);Mx1-Cre mice exposed 4 wks earlier to pIpC were analyzed for retention of the floxed Cebpa enhancer by PCR. (B) Representative FC data for transitional and mature splenic B cells from Cebpa Enh(f/f) and Cebpa Enh(f/f);Mx1-Cre mice (left), and enumeration of these subsets (right, mean and SD from four determinations). (C) Enumeration of transitional and mature splenic B cells for Enh(f/f) and Enh(f/f);Mb1-Cre mice (mean and SD from three determinations). (D) Representative FC for IgE, IgG1, and IgG2b expression in splenic B cells cultured with IL-4 and either anti-CD40 antibody or LPS for 4 days, with mean values with SD for the percentage of Ig-expressing cells from two determinations shown. (E) Serum from Enh(f/f) and Enh(f/f);Mx1-Cre mice was evaluated for IgM, IgG, and IgA by ELISA (mean and SD from three determinations).