PreproB cells that expresses a Cebpa +37 kb
enhancer/promoter transgene have B cell and myeloid potential. (A)
Diagram of the Cebpa +37 kb enhancer/promoter-hCD4 transgene
(left) and gating strategy to obtain preproB, proB, early preB, and preB cells
from these mice (right). (B) Representative CD24/hCD4 FC from
Cebpa Enh/prom-hCD4 mice (left) and the proportion of B
lineage precursors from these mice that express hCD4 (right, mean and SD from
three determinations). (C) Representative CD11b/CD19 FC after
culturing hCD4- or hCD4+ preproB cells on OP9-GFP cells
with FL and IL-7 on day 3 (D3) or day 5 (D5) and the proportion of these cells
that express CD11b or CD19 (mean and SD from three determinations).
(D) PreproB cells isolated from the bone marrows of
Cebpa Enh(f/f) or Enh(f/f);Mx1-Cre mice exposed 4 wks
earlier to pIpC were plated on OP9-GFP cells with FL and IL-7 and analyzed on
day 3 for CD11b+Gr-1- monocytes,
CD11b+Gr-1+ granulocytes, and for preproB, proB, early
preB, and preB cells (mean and SD from three determinations).