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. Author manuscript; available in PMC: 2019 Sep 4.
Published in final edited form as: Cell Metab. 2018 Jun 28;28(3):490–503.e7. doi: 10.1016/j.cmet.2018.06.001

Figure 5. Coenzyme A (CoA) rescues inhibition of M(IL-4) polarization by excess etomoxir.

Figure 5

(A) Flow cytometric analysis of CD206+/CD71+ BMDMs after 24 hr treatment with 200 μM etomoxir ± 500 μM CoA. The data shown are from one experiment representative of a total of six independent biological replicates.

(B) Aggregate data of the CD206+/CD71+ population in response to 200 μM etomoxir with or without 500 μM CoA. (n = 6 independent biological replicates)

(C) Flow cytometric analysis of BMDMs showing CD206+/CD71+ in the presence of 200 μM etomoxir and varying the concentration of added CoA. The data shown are from one experiment representative of a total of three independent biological replicates.

(D) Flow cytometric analysis of CD206+/CD71+ BMDMs in the presence of 500 μM CoA and varying the etomoxir concentration between 3-100 μM etomoxir. The data shown are from one experiment representative of a total of three independent biological replicates.

(E) Flow cytometric analysis of the CD206+/CD71+ population in WT and Cpt1a−/− BMDMs in response to IL-4 ± 200 μM etomoxir ± 500 μM CoA. The data shown are from one experiment representative of a total of four independent biological replicates.

See also Supplementary Fig. 5