Table 4.
Analysis of Glucuronides by Difference or by Direct Assays
| A. Analysis by Difference | B. Direct Analysis | Analysis of Variance | ||||||
|---|---|---|---|---|---|---|---|---|
| Nicotine Glucuronide | ||||||||
| Mean | SD | %CV | Mean | SD | %CV | F | Prob_F | |
|
|
|
|
||||||
| Pool A | 10.9 | 5.2 | 47.8 | 8.2 | 0.7 | 8.9 | 1.42 | 0.2988 |
| Pool B | 30.1 | 7.2 | 23.9 | 33.3 | 3.2 | 9.7 | 0.97 | 0.3813 |
| Pool C | 83.0 | 20.0 | 24.1 | 88.8 | 8.4 | 9.4 | 1.37 | 0.3062 |
| Pool D | 144.3 | 46.6 | 32.3 | 171.1 | 10.2 | 6.0 | 1.85 | 0.2453 |
| Pool E | 447.0 | 48.4 | 10.8 | 447.8 | 26.3 | 5.9 | 0.00 | 0.9531 |
| Cotinine Glucuronide | ||||||||
| Mean | SD | %CV | Mean | SD | %CV | F | Prob_F | |
|
|
|
|
||||||
| Pool A | 40.3 | 5.3 | 13.2 | 41.4 | 5.3 | 12.7 | 0.19 | 0.6855 |
| Pool B | 160.4 | 14.7 | 9.2 | 176.1 | 24.9 | 14.1 | 1.62 | 0.2716 |
| Pool C | 409.9 | 33.0 | 8.0 | 439.3 | 44.3 | 9.9 | 1.81 | 0.2503 |
| Pool D | 803.3 | 66.9 | 8.3 | 853.0 | 105.0 | 12.3 | 1.11 | 0.3515 |
| Pool E | 2037.0 | 121.7 | 6.0 | 2118.3 | 248.3 | 11.7 | 0.4 | 0.5605 |
| trans-3-Hydroxycotinine Glucuronide | ||||||||
| Mean | SD | %CV | Mean | SD | %CV | F | Prob_F | |
|
|
|
|
||||||
| Pool A | 38.1 | 11.3 | 29.6 | 47.1 | 6.8 | 14.4 | 6.42 | 0.0644 |
| Pool B | 158.3 | 32.7 | 20.6 | 182.9 | 28.9 | 15.8 | 2.01 | 0.2291 |
| Pool C | 364.4 | 75.0 | 20.6 | 528.9 | 131.9 | 24.9 | 4.37 | 0.1048 |
| Pool D | 769.3 | 240.4 | 31.3 | 1014.3 | 249.0 | 24.6 | 2.97 | 0.1601 |
| Pool E | 1030.4 | 356.3 | 34.6 | 1597.9 | 358.9 | 22.5 | 5.19 | 0.0850 |
Results from 6 laboratories (#1,2,3,4,5,7): 3 which used the Difference method for measuring Glucuronides (A), and 3 which measured them directly (B).
Means are expressed as ng/mL as the free base in each case
In each case, the ANOVA degrees of freedom were Source=1 and Error=5