Skip to main content
. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Stem Cells. 2018 Jul 3;36(9):1393–1403. doi: 10.1002/stem.2853

Figure 5. Hypoxic preconditioning does not inhibit osteogenic differentiation in MSC spheroids.

Figure 5

(A) ALP activity in hypoxic preconditioned 15K spheroids. (B) Calcium deposition of hypoxic preconditioned 15K spheroids; n=4. Significance is denoted by alphabetical letterings; groups with no significance are linked by the same letters, while groups with significance do not share the same letters. (C) Osteocalcin staining in alginate gels containing individual MSCs or 15K spheroids at 14 days that were preconditioned for 3 (PC3) or 0 (PC0) days and maintained in growth (GM) or osteogenic media (OM). Red arrows indicate secreted extracellular osteocalcin. Scale bar = 200 μm.