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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Clin Cancer Res. 2018 May 10;24(17):4332–4345. doi: 10.1158/1078-0432.CCR-18-0409

Figure 5.

Figure 5

LuCaP organoids maintain AR-dependence over several generations in culture. A, Adenocarcinoma and neuroendocrine organoids stained for AR (green), KRT8 (red) and DAPI (blue). B, qPCR results for expression of AR and AR-response genes. The Y axis indicates absolute copy number. The X axis represents genes tested in PDX tissue, early and late generation organoids, organoid-derived xenograft (ODX) tissue, and ODX-derived G1 organoids (ODX-G1). Error bars represent the standard error of the mean. At least two independent experiments were performed. C, Western blot for AR expression comparing PDX to multiple organoid generations. D, CellTiter Glo assay for G1, LuCaP organoids treated with 10 µM enzalutamide for two weeks. Three independent experiments were performed. E, CellTiter Glo assay for G17, LuCaP 23.1 organoids, treated by DHT withdrawal, with or without 10 µM enzalutamide for two weeks. F, CellTiter Glo assay for LuCaP 145.1 and LuCaP 145.2 organoids grown for two weeks in +DHT, −DHT, or −DHT +enzalutamide (10 µM) conditions. Error bars represent the standard error of the mean of four replicates. *P = 0.01, **P < 0.01, ***P < 0.001 determined by Student’s t-test