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. Author manuscript; available in PMC: 2019 Sep 15.
Published in final edited form as: J Immunol. 2018 Aug 13;201(6):1671–1680. doi: 10.4049/jimmunol.1800613

Figure 1. ODNps25 receptor specifically binds to phosphothioate backboned deoxyoligonucleotides.

Figure 1

(A) Intracellular uptake analysis of competitor-FITC and ODNps25-PE in co-treated human Tregs. FACS analysis was performed at 24 hrs after co-treatment of competitor-FITC and ODNps25-PE. ODNpe25, 25 base-pair length phosphorodiester backboned deoxynucleotide. Data shown is one of three independent experiments with different donors. (B) Competitive interaction between ODNps25 receptor and Biotin-ODNps25 in the presence of interfering oligonucleotides. Uptake of Biotin-ODNps25 was measured by intracellular staining with PE-conjugated streptavidin. ODNps25, 25 base-pair length phosphorothioate backboned deoxynucleotide; ODNps10, 10 base-pair length phosphorothioate backboned deoxynucleotide; ORNps25, 25 base-pair length ribonucleotide; ODNps25-biotin, ODNps25 conjugated with biotin at 5’-end. Data shown is one of three independent experiments with different donors. (C) Summarized graph of histogram shown in (B). Intracellular Biotin-ODNps25 (%) in y axis is calculated by [MFI (indicated ratio)−MFI (0:0)]/ [MFI (0:2)−MFI (0:0)] ×100. Dots indicate mean ± SD in the triplicate.