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. Author manuscript; available in PMC: 2019 Sep 4.
Published in final edited form as: Structure. 2018 Jun 14;26(9):1275–1283.e3. doi: 10.1016/j.str.2018.05.005

Figure 1. In crystallo selection of P4–P6 variants.

Figure 1

(A) Schematic of the in crystallo selection strategy. Dashed line represents iterative selections that were performed but not used in structure determination. (B) Summary of selected mutants. (C) Mutations projected on the P4–P6 secondary structure. Green boxes show mutants that are structurally characterized. Red boxes show all other mutation sites. Bases highlighted with blue background fall within 5Å of a neighboring molecule in the crystal lattice. Note that unpaired residues at the bend between P5 and P5a are drawn in a way reflecting their direct stacking on top of the P5 and P5a stems. Such drawing makes it easier to understand the structural changes induced by the M2 mutations.