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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Cancer Immunol Res. 2018 Jul 6;6(9):1008–1013. doi: 10.1158/2326-6066.CIR-18-0102

Figure 1. NGS analysis of alloHSCT Fab-phage library selection.

Figure 1

A, shown is the relative abundance of the three most frequent HCDR3 amino acid sequences before selection and the most frequent HCDR3 motif after selection of the alloHSCT Fab-phage library by three rounds of panning on CLL cells. VGR stands for VGRLQYDYYFDS; YGM stands for YGMDV; THI stands for THIVVVIPPGYFDL; GGQ stands for GGQTIDI (the HCDR3 of JML-1). Blue and red columns show the respective relative abundance before and after selection. The values on top of the paired columns give the log2 enrichment. B, amino-acid sequences of the variable light (VL) and heavy chain domains (VH) of selected post-alloHSCT Fab JML-1 (U.S. Patent 8,877,199). FR and CDR denote framework regions and complementarity-determining regions, respectively.